Evaluation of Two Primer Sets for Amplification of Comammox Nitrospira amoA Genes in Wetland Soils.

ABSTRACT: After the discovery of complete ammonia-oxidizing (comammox) Nitrospira, detection and assessments of the contribution of comammox Nitrospira communities to nitrogen cycling are in great demand. PCR-based approach, a common method for the detection of comammox, depends strongly on accurate amplification of the amoA genes from the original DNA samples using appropriate primers. In this study, we reported an evaluation of the performance of two commonly used primer sets, Ntsp-amoA 162F/359R and comaA/B-244f/659r, for amplifying the comammox amoA genes from three representative wetland soils in China [Sangsang (SS), Sanjiang (SJ), and Xianghai (XH)]. Our results demonstrated the two primer sets could both successfully amplify the clades with high relative abundance

SUBMITTER: Lin C 

PROVIDER: S-EPMC7555835 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature