Usefulness of a novel device to divide core needle biopsy specimens in a spatially matched fashion

ABSTRACT: We developed a novel dividing device that can split needle biopsy tissues along longitude axis aiming to achieve definitive molecular-biological and genetical analysis with reference of pathological diagnosis of the side-by-side divided tissue as spatially matched information. The aim of this study was to evaluate the feasibility and potential usefulness of the novel dividing device to provide the appropriate materials for molecular diagnosis. The new device was examined using mouse xenograft tumors. Real-time quantitative PCR and genetic test were performed to evaluate the feasibility and usefulness of the device. All the samples from needle biopsy were successfully divided into two pieces. Quality and quantity from divided samples harbor high enough to perform gene expression analysis (r

SUBMITTER: Shiraishi T 

PROVIDER: S-EPMC7555856 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature