Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.

ABSTRACT: In vivo bioluminescence imaging (BLI), which is based on luminescence emitted by the luciferase-luciferin reaction, has enabled continuous monitoring of various biochemical processes in living animals. Bright luminescence with a high signal-to-background ratio, ideally red or near-infrared light as the emission maximum, is necessary for in vivo animal experiments. Various attempts have been undertaken to achieve this goal, including genetic engineering of luciferase, chemical modulation of luciferin, and utilization of bioluminescence resonance energy transfer (BRET). In this review, we overview a recent advance in the development of a bioluminescence system for in vivo BLI. We also specifically examine the improvement in bioluminescence intensity by mutagenic or chemical modulation on sev

SUBMITTER: Endo M 

PROVIDER: S-EPMC7555964 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature