Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene.

ABSTRACT:

Background

The naked caryopsis character in barley is a domestication-associated trait defined by loss-of-function of the NUD gene. The functional NUD gene encodes an Apetala 2/Ethylene-Response Factor (AP2/ERF) controlling the formation of a cementing layer between pericarp and both lemma and palea. The downstream genes regulated by the NUD transcription factor and molecular mechanism of a cementing layer formation are still not sufficiently described. A naturally occurring 17-kb deletion in the nud locus is associated with the emergence of naked barley. Naked barley has been traditionally used for food and nowadays is considered as a dietary component for functional nutrition.

Results

In the present study, we demonstrate that targeted knockout of the NUD gene using RNA-gui

SUBMITTER: Gerasimova SV 

PROVIDER: S-EPMC7556925 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature