PET imaging of P2X₇R in the experimental autoimmune encephalomyelitis model of multiple sclerosis using [¹¹C]SMW139.

ABSTRACT:

Background

Non-invasive imaging of the activation status of microglia and the ability to identify a pro- or anti-inflammatory environment can provide valuable insights not only into pathogenesis of neuro-inflammatory and neurodegenerative diseases but also the monitoring of the efficacy of immunomodulatory therapies. P2X₇R is highly expressed on pro-inflammatory microglia and [¹¹C]SMW139, a specific P2X₇R tracer for positron emission tomography imaging, showed good pharmacokinetics, stability, and brain permeability in vivo. Our objective was to evaluate the potential of [¹¹C]SMW139 for PET imaging of neuroinflammation in vivo in the experimental autoimmune encephalomyelitis (EAE) model.

Methods

We induced EAE in Lewis rats by immu

SUBMITTER: Beaino W 

PROVIDER: S-EPMC7556947 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature