Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.

ABSTRACT:

Background

Mutations in TSC2 are the most common cause of tuberous sclerosis (TSC), a disorder with a high incidence of autism and intellectual disability. TSC2 regulates mRNA translation required for group 1 metabotropic glutamate receptor-dependent synaptic long-term depression (mGluR-LTD) and behavior, but the identity of mRNAs responsive to mGluR-LTD signaling is largely unknown.

Methods

We utilized Tsc2^+/- mice as a mouse model of TSC and prepared hippocampal slices from these animals. We induced mGluR-LTD synaptic plasticity in slices and processed the samples for RNA-seq and ribosome profiling to identify differentially expressed genes in Tsc2^+/- and following mGluR-LTD synaptic plasticity.

Results

Ribosome profiling reveals that in Tsc2

SUBMITTER: Hien A 

PROVIDER: S-EPMC7556950 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature