Morphological and molecular characterization of Sarcocystis wenzeli in chickens (Gallus gallus) in China.

ABSTRACT:

Background

There has been considerable confusion concerning the number and classification of Sarcocystis spp. in chickens. Scarce nucleotide data of Sarcocystis spp. from chickens are provided in GenBank. The study aimed to investigate the morphological and molecular characteristics of Sarcocystis spp. found in chickens in China.

Methods

Tissues from 33 chickens were collected in 2019. Sarcocysts were observed using light (LM) and transmission electron microscopy (TEM). Individual sarcocysts from different chickens were selected for DNA extraction, and five loci, 18S rDNA, 28S rDNA, ITS1 region, the mitochondrial cox1 gene and the apicoplastic rpoB gene, were amplified from each sarcocyst, sequenced and analyzed.

Results

Only S. wenzeli was found in 14 of 33 (42.4%) c

SUBMITTER: Pan J 

PROVIDER: S-EPMC7557040 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature