Metabolome analysis using multiple data mining approaches suggests luteolin biosynthesis in Physcomitrella patens.

ABSTRACT: The model land plant Physcomitrella patens synthesizes flavonoids which may act as protectant of ultraviolet-B radiation. We aimed to uncover its flavonoid profile, for which metabolome analysis using liquid chromatography coupled with Ion trap/Orbitrap mass spectrometry was performed. From the 80% methanol extracts, 661 valid peaks were detected. Prediction of the elemental compositions within a mass accuracy of 2 ppm indicated that 217 peaks had single elemental composition. A compound database search revealed 47 peaks to be annotated as secondary metabolites based on the compound database search. Comprehensive substituent search by ShiftedIonsFinder showed there were 13 peaks of potential flavonoid derivatives. Interestingly, a peak having m/z 287.0551, correspondin

SUBMITTER: Hiraga Y 

PROVIDER: S-EPMC7557656 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature