Meso-Substituted Thiazole Orange for Selective Fluorescence Detection to G-Quadruplex DNA and Molecular Docking Simulation.

ABSTRACT: The cyanine dye thiazole orange (TO, including 2TO and 4TO) is widely used as a light-up fluorescent probe upon binding to almost all forms of DNA, but it exhibits poor selectivity for recognizing G-quadruplex DNA (G-DNA), which has significant biological functions in biological processes and therapeutic applications. Here, introducing benzyl substituent to the meso position of the methine chain of 2TO is expected to selectively recognize G-DNA. The spectroscopic titrations reveal that modified 2TO (meso-Bn-2TO) has almost no background fluorescence in solution and shows a preference to bind with G-DNA over ssDNA, dsDNA, and ct-DNA. Specifically, meso-Bn-2TO 1a displays a strong fluorescent signal upon interaction with G-DNA and a very weak fluorescent signal up

SUBMITTER: Guan L 

PROVIDER: S-EPMC7557940 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature