Rearing and Maintenance of Galleria mellonella and Its Application to Study Fungal Virulence.

ABSTRACT: Galleria mellonella larvae have been widely used as alternative non-mammalian models for the study of fungal virulence and pathogenesis. The larvae can be acquired in small volumes from worm farms, pet stores, or other independent suppliers commonly found in the United States and parts of Europe. However, in countries with no or limited commercial availability, the process of shipping these larvae can cause them stress, resulting in decreased or altered immunity. Furthermore, the conditions used to rear these larvae including diet, humidity, temperature, and maintenance procedures vary among the suppliers. Variation in these factors can affect the response of G. mellonella larvae to infection, thereby decreasing the reproducibility of fungal virulence experiments. There is a critical need

SUBMITTER: Firacative C 

PROVIDER: S-EPMC7558789 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature