MiR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.

ABSTRACT:

Background

The asymptomatic onset, frequent recurrence, and poor prognosis of hepatocellular carcinoma (HCC) prompted us to identify new therapeutic targets or predictive markers of HCC diagnosis or prognosis.

Methods

In this study, bioinformatics analysis was used to screen for target miRNAs from the open-access TCGA database. Transwell assays, Western blotting, and qRT-PCR analyses were used to detect cellular functions and gene expression in HCC cells and samples. A nude mouse tumorigenesis model was established to facilitate the observation of HCC progression. Other assays included luciferase reporter assays, IHC, and survival analysis.

Results

We found that the identified miR-876 from TCGA was expressed at low levels in HCC cell lines and that low miR-876 express

SUBMITTER: Chen K 

PROVIDER: S-EPMC7559219 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature