Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture.

ABSTRACT: The three-dimensional architecture of the genome plays an essential role in establishing and maintaining cell identity. However, the magnitude and temporal kinetics of changes in chromatin structure that arise during cell differentiation remain poorly understood. Here, we leverage a murine model of erythropoiesis to study the relationship between chromatin conformation, the epigenome, and transcription in erythroid cells. We discover that acute transcriptional responses induced by erythropoietin (EPO), the hormone necessary for erythroid differentiation, occur within an invariant chromatin topology. Within this pre-established landscape, Yin Yang 1 (YY1) occupancy dynamically redistributes to sites in proximity of EPO-regulated genes. Using HiChIP, we identify chromatin contacts mediated b

SUBMITTER: Perreault AA 

PROVIDER: S-EPMC7559257 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature