Selective Eradication of Staphylococcus aureus by the Designer Genetically Programmed Yeast Biocontrol Agent.

ABSTRACT: Staphylococcus aureus is a common human pathogen that is particularly often associated with antibiotic resistance. The eradication of this ubiquitous infectious agent from its ecological niches and contaminated surfaces is especially complicated by excessive biofilm formation and persisting cells, which evade the antibacterial activity of conventional antibiotics. Here, we present an alternative view of the problem of specific S. aureus eradication. The constitutive heterologous production of highly specific bacteriolytic protease lysostaphin in yeast Pichia pastoris provides an efficient biocontrol agent, specifically killing S. aureus in coculture. A yeast-based anti-S. aureus probiotic was efficient in a high range of temperatures and target-to-effector ratios, indicating its robustness

SUBMITTER: Pipiya SO 

PROVIDER: S-EPMC7559405 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature