Diesel exhaust particle exposure reduces expression of the epithelial tight junction protein Tricellulin.

ABSTRACT:

Background

While exposure to diesel exhaust particles has been linked to aberrant immune responses in allergic diseases such as asthma, little attention has been paid to their effects on the airway epithelial barrier. In this study, we sought to determine the effect of diesel exhaust exposure on airway epithelial barrier function and composition using in vitro and in vivo model systems.

Methods

16HBE14o- human bronchial epithelial cells were grown on collagen coated Transwell inserts and exposed to 5 to 50 μg/cm² SRM 2975 diesel particulate matter (DEP) suspended in cell culture medium or vehicle controls. Changes in barrier function were assessed by measuring transepithelial electrical resistance (TEER) and permeability to 4 kDa FITC Dextran. Neonatal BALB/c mice

SUBMITTER: Smyth T 

PROVIDER: S-EPMC7560077 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature