Development of a novel and rapid phenotype-based screening method to assess rice seedling growth.

ABSTRACT:

Background

Rice (Oryza sativa) is one of the most important model crops in plant research. Despite its considerable advantages, (phenotypic) bioassays for rice are not as well developed as for Arabidopsis thaliana. Here, we present a phenotype-based screening method to study shoot-related parameters of rice seedlings via an automated computer analysis.

Results

The phenotype-based screening method was validated by testing several compounds in pharmacological experiments that interfered with hormone homeostasis, confirming that the assay was consistent with regard to the anticipated plant growth regulation and revealing the robustness of the set-up in terms of reproducibility. Moreover, abiotic stress tests using NaCl and DCMU, an electron transport blocker durin

SUBMITTER: Vlaminck L 

PROVIDER: S-EPMC7560306 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature