A genotyping method combining primer competition PCR with HRM analysis to identify point mutations in Duchenne animal models.

ABSTRACT: Dystrophin-null sapje zebrafish is an excellent model for better understanding the pathological mechanisms underlying Duchenne muscular dystrophy, and it has recently arisen as a powerful tool for high-throughput screening of therapeutic candidates for this disease. While dystrophic phenotype in sapje larvae can be easily detected by birefringence, zebrafish genotyping is necessary for drug screening experiments, where the potential rescue of larvae phenotype is the primary outcome. Genotyping is also desirable during colony husbandry since heterozygous progenitors need to be selected. Currently, sapje zebrafish are genotyped through techniques involving sequencing or multi-step PCR, which are often costly, tedious, or require special equipment. Here we report a simple, precise, cost-effec

SUBMITTER: Lasa-Fernandez H 

PROVIDER: S-EPMC7560699 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature