Conformational equilibrium shift underlies altered K⁺ channel gating as revealed by NMR.

ABSTRACT: The potassium ion (K⁺) channel plays a fundamental role in controlling K⁺ permeation across the cell membrane and regulating cellular excitabilities. Mutations in the transmembrane pore reportedly affect the gating transitions of K⁺ channels, and are associated with the onset of neural disorders. However, due to the lack of structural and dynamic insights into the functions of K⁺ channels, the structural mechanism by which these mutations cause K⁺ channel dysfunctions remains elusive. Here, we used nuclear magnetic resonance spectroscopy to investigate the structural mechanism underlying the decreased K⁺-permeation caused by disease-related mutations, using the prokaryotic K⁺ channel KcsA. We demonstrated that the

SUBMITTER: Iwahashi Y 

PROVIDER: S-EPMC7560842 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature