Enzyme immunoassays for detection and quantification of venoms of Sri Lankan snakes: Application in the clinical setting.

ABSTRACT:

Background

Detection and quantification of snake venom in envenomed patients' blood is important for identifying the species responsible for the bite, determining administration of antivenom, confirming whether sufficient antivenom has been given, detecting recurrence of envenoming, and in forensic investigation. Currently, snake venom detection is not available in clinical practice in Sri Lanka. This study describes the development of enzyme immunoassays (EIA) to differentiate and quantify venoms of Russell's viper (Daboia russelii), saw-scaled viper (Echis carinatus), common cobra (Naja naja), Indian krait (Bungarus caeruleus), and hump-nosed pit viper (Hypnale hypnale) in the blood of envenomed patients in Sri Lanka.

Methodology / principal findings

A double sandwich EIA

SUBMITTER: Maduwage KP 

PROVIDER: S-EPMC7561112 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature