Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.

ABSTRACT: All Neisseria gonorrhoeae strains contain multiple copies of integrated filamentous phage genomes with undefined structures. In this study, we sought to characterize the capsid proteins of filamentous N. gonorrhoeae bacteriophage NgoΦ6 and phagemids propagated in different bacteria. The data demonstrate that purified phage contain phage-encoded structural proteins and bacterial host proteins; host proteins consistently copurified with the phage particles. The bacterial host proteins associated with the phage filament (as identified by mass spectrometry) tended to be one of the predominant outer membrane components of the host strain, plus minor additional host proteins. We were able to copurify a functional ß-lactamase, a phagemid-encoded protein, with phage filaments. We used protein mode

SUBMITTER: Piekarowicz A 

PROVIDER: S-EPMC7561177 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature