Establishment of a relationship between blastomere geometry and YAP localisation during compaction.

ABSTRACT: Precise patterning within the three-dimensional context of tissues, organs and embryos implies that cells can sense their relative position. During preimplantation development, outside and inside cells rely on apicobasal polarity and the Hippo pathway to choose their fate. Despite recent findings suggesting that mechanosensing might be central to this process, the relationship between blastomere geometry (i.e. shape and position) and the Hippo pathway effector YAP remains unknown. We used a highly quantitative approach to analyse information on the geometry and YAP localisation of individual blastomeres of mouse and human embryos. We identified the proportion of exposed cell surface area as most closely correlating with the nuclear localisation of YAP. To test this relationship, we develop

SUBMITTER: Royer C 

PROVIDER: S-EPMC7561472 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature