Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.

ABSTRACT: Precisely altering gene expression is critical for understanding molecular processes of embryogenesis. Although some tools exist for transgene misexpression in developing chick embryos, we have refined and advanced them by simplifying and optimizing constructs for spatiotemporal control. To maintain expression over the entire course of embryonic development we use an enhanced piggyBac transposon system that efficiently integrates sequences into the host genome. We also incorporate a DNA targeting sequence to direct plasmid translocation into the nucleus and a D4Z4 insulator sequence to prevent epigenetic silencing. We designed these constructs to minimize their size and maximize cellular uptake, and to simplify usage by placing all of the integrating sequences on a single plasmid. F

SUBMITTER: Chu D 

PROVIDER: S-EPMC7561481 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature