Effect of DNMT3A polymorphisms on CpG island hypermethylation in gastric mucosa.

ABSTRACT:

Background

CpG methylation of tumor suppressor genes occurs in the early stage of carcinogenesis. Detecting risk factors for aberrant CpG methylation is clinically important for predicting cancer development. DNA methyltransferase (DNMT) 3a is considered to play critical roles in the DNA methylation process during pathogenesis. In this study, we evaluated the association between DNMT3A polymorphisms (rs6733868 and rs13428812) and CpG methylation status in non-cancerous gastric mucosa.

Methods

We determined the DNMT3A genotype and CpG methylation status of 4 genes (p14^ARF, p16^INK4a, DAPK, and CDH1) in 510 subjects without gastric cancer. Helicobacter pylori (HP) infection status was determined by the rapid urease test, urea breath test, speculum examinat

SUBMITTER: Takano H 

PROVIDER: S-EPMC7562764 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature