Liver Transcriptome Responses to Heat Stress and Newcastle Disease Virus Infection in Genetically Distinct Chicken Inbred Lines.

ABSTRACT: Heat stress results in reduced productivity, anorexia, and mortality in chickens. The objective of the study was to identify genes and signal pathways associated with heat stress and Newcastle disease virus (NDV) infection in the liver of chickens through RNA-seq analysis, using two highly inbred chicken lines (Leghorn and Fayoumi). All birds were held in the same environment until 14 days of age. On day 14, half the birds were exposed to 38 °C with 50% relative humidity for 4 h, then 35 °C until the end of the experiment. The remaining birds were kept at 25 °C throughout the experiment. The heat-treated birds were inoculated at 21 days of age with 10⁷ EID₅₀ (One EID₅₀ unit is the amount of virus that will infect 50 percent of inoculated embryos) NDV La Sot

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7563548 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature