The Cytokine IL-1β and Piperine Complex Surveyed by Experimental and Computational Molecular Biophysics.

ABSTRACT: The bioactive piperine, a compound found in some pepper species, has been widely studied because of its therapeutic properties that include the inhibition of an important inflammation pathway triggered by interleukin-1 beta (IL-1β). However, investigation into the molecular interactions between IL-1β and piperine is not reported in the literature. Here, we present for the first time the characterisation of the complex formed by IL-1β and piperine through experimental and computational molecular biophysical analyses. Fluorescence spectroscopy unveiled the presence of one binding site for piperine with an affinity constant of 14.3 × 10⁴ M^-1 at 298 K. The thermodynamic analysis indicated that the interaction with IL-1β was spontaneous (∆G = -25 kJ/mol) and, when split in

SUBMITTER: Zazeri G 

PROVIDER: S-EPMC7563551 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature