LvYY1 Activates WSSV ie1 Promoter for Enhanced Vaccine Production and Efficacy.

ABSTRACT: The baculovirus expression vector system (BEVS) has been used as a preferred platform for the production of recombinant protein complexes and efficacious vaccines. However, limited protein yield hinders the application of BEVS. It is well accepted that transcription enhancers are capable of increasing translational efficiency of mRNAs, thereby achieving better protein production. In this study, the ability of LvYY1 as a transcription enhancer was assessed. LvYY1 could interact with the WSSV ie1 promoter via binding to special DNA sites in BEVS. The effects of LvYY1 on protein expression mediated by WSSV ie1 promoter of BEVS was investigated using eGFP as a reporter gene. Enhanced eGFP expression was observed in Sf-9 cells with LvYY1. On this basis,

SUBMITTER: Tao LN 

PROVIDER: S-EPMC7563808 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature