Haploidy in Tobacco Induced by PsASGR-BBML Transgenes via Parthenogenesis.

ABSTRACT:

Background

Engineering apomixis in sexually reproducing plants has been long desired because of the potential to fix hybrid vigor. Validating the functionality of genes originated from apomictic species that contribute to apomixis upon transfer to sexually reproducing species is an important step. The PsASGR-BABYBOOM-like (PsASGR-BBML) gene from Pennisetum squamulatum confers parthenogenesis in this apomict, and its functionality was demonstrated in several sexually reproducing monocots but not in any dicots.

Methods

We introduced the PsASGR-BBML gene regulated by egg cell-specific promoters, either AtDD45 or AtRKD2, into tobacco, and analyzed progeny of the transgenic lines resulting from self-pollination and crossing by flow cytome

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC7564442 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature