Multivalent and Bidirectional Binding of Transcriptional Transactivation Domains to the MED25 Coactivator.

ABSTRACT: The human mediator subunit MED25 acts as a coactivator that binds the transcriptional activation domains (TADs) present in various cellular and viral gene-specific transcription factors. Previous studies, including on NMR measurements and site-directed mutagenesis, have only yielded low-resolution models that are difficult to refine further by experimental means. Here, we apply computational molecular dynamics simulations to study the interactions of two different TADs from the human transcription factor ETV5 (ERM) and herpes virus VP16-H1 with MED25. Like other well-studied coactivator-TAD complexes, the interactions of these intrinsically disordered domains with the coactivator surface are temporary and highly dynamic ('fuzzy'). Due to the fact that the MED25 TAD-binding region is organi

SUBMITTER: Jeffery HM 

PROVIDER: S-EPMC7564715 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature