Control of Transcription Initiation by Biased Thermal Fluctuations on Repetitive Genomic Sequences.

ABSTRACT: In the process of transcription initiation by RNA polymerase, promoter DNA sequences affect multiple reaction pathways determining the productivity of transcription. However, the question of how the molecular mechanism of transcription initiation depends on the sequence properties of promoter DNA remains poorly understood. Here, combining the statistical mechanical approach with high-throughput sequencing results, we characterize abortive transcription and pausing during transcription initiation by Escherichia coli RNA polymerase at a genome-wide level. Our results suggest that initially transcribed sequences, when enriched with thymine bases, contain the signal for inducing abortive transcription, whereas certain repetitive sequence elements embedded in promoter regions constitute

SUBMITTER: Imashimizu M 

PROVIDER: S-EPMC7564750 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature