Detection and discrimination of influenza B Victoria lineage deletion variant viruses by real-time RT-PCR.

ABSTRACT: BackgroundDuring the 2016/17 influenza season, influenza B/VIC lineage variant viruses emerged with two (K₁₆₂N₁₆₃) or three (K₁₆₂N₁₆₃D₁₆₄) amino acid (aa) deletions in the haemagglutinin (HA) protein. There are currently five antigenically distinct HA proteins expressed by co-circulating influenza B viruses: B/YAM, B/VIC V1A (no deletion), B/VIC V1A-2DEL (2 aa deletion) and two antigenically distinguishable groups of B/VIC V1A-3DEL (3 aa deletion). The prevalence of these viruses differs across geographical regions, making it critical to have a sensitive, rapid diagnostic assay that detects and distinguishes these influenza B variant viruses during surveillance.AimOur objective was to develop a real-time RT-PCR (rRT-PCR) assay for dete

SUBMITTER: Shu B 

PROVIDER: S-EPMC7565853 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature