Phage-DMS: A Comprehensive Method for Fine Mapping of Antibody Epitopes.

ABSTRACT: Understanding the antibody response is critical to developing vaccine and antibody-based therapies and has inspired the recent development of new methods to isolate antibodies. Methods to define the antibody-antigen interactions that determine specificity or allow escape have not kept pace. We developed Phage-DMS, a method that combines two powerful approaches-immunoprecipitation of phage peptide libraries and deep mutational scanning (DMS)-to enable high-throughput fine mapping of antibody epitopes. As an example, we designed sequences encoding all possible amino acid variants of HIV Envelope to create phage libraries. Using Phage-DMS, we identified sites of escape predicted using other approaches for four well-characterized HIV monoclonal antibodies with known linear epitopes. In some ca

SUBMITTER: Garrett ME 

PROVIDER: S-EPMC7566095 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature