Ribo-Pop: simple, cost-effective, and widely applicable ribosomal RNA depletion.

ABSTRACT: The measurement of RNA abundance derived from massively parallel sequencing experiments is an essential technique. Methods that reduce ribosomal RNA levels are usually required prior to sequencing library construction because ribosomal RNA typically comprises the vast majority of a total RNA sample. For some experiments, ribosomal RNA depletion is favored over poly(A) selection because it offers a more inclusive representation of the transcriptome. However, methods to deplete ribosomal RNA are generally proprietary, complex, inefficient, applicable to only specific species, or compatible with only a narrow range of RNA input levels. Here, we describe Ribo-Pop (ribosomal RNA depletion for popular use), a simple workflow and antisense oligo design strategy that we demonstrate works over a wi

SUBMITTER: Thompson MK 

PROVIDER: S-EPMC7566562 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature