Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing.

ABSTRACT: MicroRNA expression is important for gene regulation and deregulated microRNA expression is often observed in diseases such as cancer. The processing of primary microRNA transcripts is an important regulatory step in microRNA biogenesis. Due to low expression level and association with chromatin, primary microRNAs are challenging to study in clinical samples where input material is limited. Here, we present a high-sensitivity targeted method to determine processing efficiency of several hundred primary microRNAs from total RNA that requires relatively few RNA sequencing reads. We validate the method using RNA from HeLa cells and show the applicability to clinical samples by analyzing RNA from normal liver and hepatocellular carcinoma. We identify 24 primary microRNAs with significant chang

SUBMITTER: Conrad T 

PROVIDER: S-EPMC7566579 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature