Efavirenz induces DNA damage response pathway in lung cancer.

ABSTRACT: The cell-cycle related genes are potential gene targets in understanding the effects of efavirenz (EFV) in lung cancer. The present study aimed at investigating the expression changes of cell-cycle related genes in response to EFV drug treatment in human non-small cell lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. The loss in nuclear integrity in response to EFV was detected by 4', 6-diamidino-2-phenylindole (DAPI) staining. Gene expression profiling was performed using human cell cycle PathwayFinder RT² Profiler™ PCR Array. The expression changes of 84 genes key to the cell cycle pathway in humans following EFV treatment was examined. The R² PCR Array analysis revealed a change in expression of selected gene targets (including MAD2L2, CASP3, AURKB).

SUBMITTER: Marima R 

PROVIDER: S-EPMC7566803 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature