Systematic monitoring of glanders-infected horses by complement fixation test, bacterial isolation, and PCR.

ABSTRACT: Glanders is an equine zoonosis caused by Burkholderia mallei that is responsible for considerable economic loss. Complement fixation testing (CFT) using warm or cold incubation are recommended by the OIE, but many routinely used detection tests may present misleading results. To increase accuracy of glanders diagnosis and establish an appropriate protocol in collaboration with the National Equine Health Program, seven horses positive for glanders kept in isolation in Brazil were examined fortnightly by CFT, microbiological screening, and molecular testing. Warm and cold serologies with USDA and c.c.Pro antigens, respectively, were performed on 132 samples using the US Department of Agriculture protocol. The warm and cold serologies showed, respectively,12.9% and 17.3% serorea

SUBMITTER: Abreu DC 

PROVIDER: S-EPMC7566944 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature