Age-related decline of interferon-gamma responses in macrophage impairs satellite cell proliferation and regeneration.

ABSTRACT:

Background

Impaired muscle regeneration and increased muscle fibrosis are observed in aged muscle accompanied by progressive loss of muscle mass (sarcopenia). However, the underlying mechanism is still unclear.

Methods

The differentiated expressed genes in young and aged muscles after acute injury by cardiotoxin were identified by RNA-sequence analysis. Single-cell RNA-sequence analysis was used to identify cell clusters and functions in young muscle after acute injury, and flow cytometry analysis and sorting were used to validate the function. The proliferation and differentiation functions of satellite cells were accessed by immunostaining with 5-ethynyl-2'-deoxyuridine and embryonic myosin heavy chain (eMyHC), respectively. Muscle regeneration ability was accessed by hist

SUBMITTER: Zhang C 

PROVIDER: S-EPMC7567146 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature