Label-Free in Situ pH Monitoring in a Single Living Cell Using an Optical Nanoprobe.

ABSTRACT: Intracellular pH plays critical roles in cell and tissue functions during processes such as metabolism, proliferation, apoptosis, ion transportation, endocytosis, muscle contraction and so on. It is thus an important biomarker that can readily be used to monitor the physiological status of a cell. Thus, disrupted intracellular pH may serve as an early indicator of cell dysfunction and deterioration. Various methods have been developed to detect cellular pH, such as pH-sensitive labeling reagents with fluorescent or Raman signals. However, excessive cellular uptake of these reagents will not only disrupt cell viability but also compromise effective long-term monitoring. Here, we present a novel fiber-optic fluorescent nanoprobe with a high spatial resolution for label-free, subcellular pH s

SUBMITTER: Yang Q 

PROVIDER: S-EPMC7567407 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature