Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities.

ABSTRACT: The world is currently facing a novel viral pandemic (SARS-CoV-2), and large-scale testing is central to decision-making for the design of effective policies and control strategies to minimize its impact on the global population. However, testing for the presence of the virus is a major bottleneck in tracking the spreading of the disease. Given its adaptability regarding the nucleotide sequence of target regions, RT-qPCR is a strong ally to reveal the rapid geographical spreading of novel viruses. We assessed PCR variations in the SARS-CoV-2 diagnosis taking into account public genome sequences and diagnosis kits used by different countries. We analyzed 226 SARS-CoV-2 genome sequences from samples collected by March 22, 2020. Our work utilizes a phylogenetic approach that reveals the early

SUBMITTER: Barreto HG 

PROVIDER: S-EPMC7567654 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature