Nanosecond pulsed electrical fields enhance product recovery in plant cell fermentation.

ABSTRACT: The potential of pharmacologically active secondary plant metabolites is limited by the low yield from often rare plants, and the lack of economically feasible chemical synthesis of these complex compounds. Plant cell fermentation offers an alternative strategy to overcome these constraints. However, the efficiency of this approach is limited by intracellular sequestration of the products, such that continuous bioprocessing is not possible. As a precondition for such a, more attractive, continuous process, it is of great importance to stimulate the export of the product into the medium without impairing viability and, thus, the productivity of the cells. Using nicotine alkaloids of tobacco as a case study, an alternative strategy is explored, where nanosecond pulsed electric fields (nsPEFs

SUBMITTER: Rajabi F 

PROVIDER: S-EPMC7567687 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature