Multiscale fluorescent tracking of immune cells in the liver with a highly biocompatible far-red emitting polymer probe.

ABSTRACT: The development of innovative immune cell therapies relies on efficient cell tracking strategies. For this, multiscale fluorescence-based analyses of transferred cells into the host with complementary techniques, including flow cytometry for high-throughput cell analysis and two-photon microscopy for deep tissue imaging would be highly beneficial. Ideally, cells should be labelled with a single fluorescent probe combining all the properties required for these different techniques. Due to the intrinsic autofluorescence of most tissues and especially the liver, far-red emission is also an important asset. However, the development of far-red emitting probes suitable for two-photon microscopy and compatible with clearing methods to track labelled immune cells in thick samples, remains challeng

SUBMITTER: Daniel M 

PROVIDER: S-EPMC7567820 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature