Kinetic Analysis of AAA+ Translocases by Combined Fluorescence and Anisotropy Methods.

ABSTRACT: The multitude of varied, energy-dependent processes that exist in the cell necessitate a diverse array of macromolecular machines to maintain homeostasis, allow for growth, and facilitate reproduction. ATPases associated with various cellular activity are a set of protein assemblies that function as molecular motors to couple the energy of nucleoside triphosphate binding and hydrolysis to mechanical movement along a polymer lattice. A recent boom in structural insights into these motors has led to structural hypotheses on how these motors fulfill their function. However, in many cases, we lack direct kinetic measurements of the dynamic processes these motors undergo as they transition between observed structural states. Consequently, there is a need for improved techniques for testing the

SUBMITTER: Scull NW 

PROVIDER: S-EPMC7567984 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature