Single-Color Fluorescence Lifetime Cross-Correlation Spectroscopy In Vivo.

ABSTRACT: The ability to quantify protein concentrations and to measure protein interactions in vivo is key information needed for the understanding of complex processes inside cells, but the acquisition of such information from living cells is still demanding. Fluorescence-based methods like two-color fluorescence cross-correlation spectroscopy can provide this information, but measurement precision is hampered by various sources of errors caused by instrumental or optical limitations such as imperfect overlap of detection volumes or detector cross talk. Furthermore, the nature and properties of used fluorescent proteins or fluorescent dyes, such as labeling efficiency, fluorescent protein maturation, photostability, bleaching, and fluorescence brightness can have an impact. Here, we take advantage

SUBMITTER: Stefl M 

PROVIDER: S-EPMC7568003 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature