P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response.

ABSTRACT:

Objective

Diabetic nephropathy (DN) is one of the most common complications of diabetes and a critical risk factor for developing end-stage renal disease. Activation of purinergic receptors, including P2Y2R has been associated with the pathogenesis of renal diseases, such as polycystic kidney and glomerulonephritis. However, the role of P2Y2R and its precise mechanisms in DN remain unknown. We hypothesised that P2Y2R deficiency may play a protective role in DN by modulating the autophagy signalling pathway.

Methods

We used a mouse model of DN by combining a treatment of high-fat diet and streptozotocin after unilateral nephrectomy in wild-type or P2Y2R knockout mice. We measured renal functional parameter in plasma, examined renal histology, and analysed expression of autoph

SUBMITTER: Dusabimana T 

PROVIDER: S-EPMC7568185 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature