Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions.

ABSTRACT:

Background

A new Bacillus thuringiensis X023 (BtX023) with high insecticidal activity was isolated in Hunan Province, China. The addition of metals (Cu, Fe, Mg and Mn) to the medium could influence the formation of spores and/or insecticidal crystal proteins (ICPs). In previous studies, Cu ions considerably increased the synthesis of ICPs by enhancing the synthesis of poly-β-hydroxy butyrate. However, the present study could provide new insights into the function of Cu ions in ICPs.

Results

Bioassay results showed that wild strain BtX023 exhibited high insecticidal activity against Plutella xylostella. The addition of 1 × 10^-5 M Cu²⁺ could considerably increase the expression of cry1Ac and vip3Aa, and the insecticidal activity was enhanced. Quantitative

SUBMITTER: Liu Z 

PROVIDER: S-EPMC7568400 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature