Standardization and harmonization of distributed multi-center proteotype analysis supporting precision medicine studies.

ABSTRACT: Cancer has no borders: Generation and analysis of molecular data across multiple centers worldwide is necessary to gain statistically significant clinical insights for the benefit of patients. Here we conceived and standardized a proteotype data generation and analysis workflow enabling distributed data generation and evaluated the quantitative data generated across laboratories of the international Cancer Moonshot consortium. Using harmonized mass spectrometry (MS) instrument platforms and standardized data acquisition procedures, we demonstrate robust, sensitive, and reproducible data generation across eleven international sites on seven consecutive days in a 24/7 operation mode. The data presented from the high-resolution MS1-based quantitative data-independent acquisition (HRMS1-DIA) w

SUBMITTER: Xuan Y 

PROVIDER: S-EPMC7568553 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature