Non-invasive detection of EGFR mutations by cell-free loop-mediated isothermal amplification (CF-LAMP).

ABSTRACT: Targeting epidermal growth factor receptor (EGFR) through tyrosine kinase inhibitors (TKI) is a successful therapeutic strategy in non-small cell lung cancer. However, the response to TKI therapy depends on specific activating and acquired mutations in the tyrosine kinase domain of the EGFR gene. Therefore, confirming the EGFR status of patients is crucial, not only for determining the eligibility, but also for monitoring the emergence of mutations in patients under TKI therapy. In this study, our aim was to develop a cost effective, yet sensitive, technique that allows the detection of therapeutically-relevant EGFR hotspot mutations at isothermal conditions in a non-invasive manner. Previously, we developed an allele-specific loop-mediated isothermal amplification (AS-LAMP) assay for scre

SUBMITTER: Arjuna S 

PROVIDER: S-EPMC7568567 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature