Nature-inspired topographies on hydroxyapatite surfaces regulate stem cells behaviour.

ABSTRACT: Surface topography is one of the key factors in regulating interactions between materials and cells. While topographies presented to cells in vivo are non-symmetrical and in complex shapes, current fabrication techniques are limited to replicate these complex geometries. In this study, we developed a microcasting technique and successfully produced imprinted hydroxyapatite (HAp) surfaces with nature-inspired (honeycomb, pillars, and isolated islands) topographies. The in vitro biological performance of the developed non-symmetrical topographies was evaluated using adipose-derived stem cells (ADSCs). We demonstrated that ADSCs cultured on all HAp surfaces, except honeycomb patterns, presented well-defined stress fibers and expressed focal adhesion protein (paxillin) molecules.

SUBMITTER: Ramaswamy Y 

PROVIDER: S-EPMC7569262 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature