Retinoic Acid and Lymphotoxin Signaling Promote Differentiation of Human Intestinal M Cells.

ABSTRACT:

Background & aims

Intestinal microfold (M) cells are a unique subset of intestinal epithelial cells in the Peyer's patches that regulate mucosal immunity, serving as portals for sampling and uptake of luminal antigens. The inability to efficiently develop human M cells in cell culture has impeded studies of the intestinal immune system. We aimed to identify signaling pathways required for differentiation of human M cells and establish a robust culture system using human ileum enteroids.

Methods

We analyzed transcriptome data from mouse Peyer's patches to identify cell populations in close proximity to M cells. We used the human enteroid system to determine which cytokines were required to induce M-cell differentiation. We performed transcriptome, immunofluorescence, scanning

SUBMITTER: Ding S 

PROVIDER: S-EPMC7569531 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature