Colistin Heteroresistance among Extended Spectrum β-lactamases-Producing Klebsiella pneumoniae.

ABSTRACT: Colistin-heteroresistant (CST-HR) Enterobacterales isolates have been identified recently, challenging the clinical laboratories since routine susceptibility tests fail to detect this phenotype. In this work we describe the first CST-HR phenotype in extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolates in South America. Additionally, we determine the genomic mechanisms of colistin heteroresistance in these strains. The CST-HR phenotype was analyzed by the population analysis profile (PAP) method, and mutations associated with this phenotype were determined by whole-genome sequencing (WGS) and the local BLAST+ DB tool. As a result, 8/60 isolates were classified as CST-HR according to the PAP method. From WGS, we determined that the CST-HR isolates belong to three dif

SUBMITTER: Morales-Leon F 

PROVIDER: S-EPMC7569871 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature