Fast and Inexpensive Phenotyping and Genotyping Methods for Evaluation of Barley Mutant Population.

ABSTRACT: To further develop barley breeding and genetics, more information on gene functions based on the analysis of the mutants of each gene is needed. However, the mutant resources are not as well developed as the model plants, such as Arabidopsis and rice. Although genome editing techniques have been able to generate mutants, it is not yet an effective method as it can only be used to transform a limited number of cultivars. Here, we developed a mutant population using 'Mannenboshi', which produces good quality grains with high yields but is susceptible to disease, to establish a Targeting Induced Local Lesions IN Genomes (TILLING) system that can isolate mutants in a high-throughput manner. To evaluate the availability of the prepared 8043 M3 lines, we investigated the frequency of mutant occu

SUBMITTER: Kawamoto Y 

PROVIDER: S-EPMC7569886 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature