Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel.

ABSTRACT: The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electr

SUBMITTER: Ye Y 

PROVIDER: S-EPMC7570009 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature